DNA Extraction

Hangátirat Automatikusan generált

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  Welcome to breaking news.

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  Today. We are going to be extracting D N a.

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  As part of a crime scene investigation on breaking news.

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  So first, our first victim is the banana and our

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  first, our other victims of strawberry.

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  Well, we don't know quite who who's the victim and

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  who actually committed the crime.

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  That's why we're testing and extracting that DNA today.

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  Well, we're not gonna be testing it.

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  We're just extracting it for the the other scientists.

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  So first, we're gonna get started to remove the banana

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  from the bag and to test the DNA of the

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  banana, we have to destroy the banana.

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  We have to puree it.

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  But first, let's go over our supplies that we're gonna

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  be using.

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  Okay. So, banana, That's already said here it is.

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  Pretty average half banana, the other half.

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  It's back at the crime.

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  We walked in there.

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  Strawberry. We have one.

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  Stubborn. We cut the top off of it.

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  Or did the banana do it?

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  That's right.

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  You had to decide and then six to know.

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  Next we have some.

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  Okay, These are called test tubes, and then the test

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  tubes are sitting in the flask, so I don't have

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  to be an eight armed person.

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  It's just too much work alone I have.

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  This is called a beaker.

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  Not to be confused with the Muppet called beaker, who

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  is a scientist.

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  Next, we have some soap.

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  I like the soap.

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  The soap is pretty cool.

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  This soap smells island fresh.

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  Does that make a difference?

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  We'll find out.

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  Then we have our salt and we have rubbing.

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  Alcohol is currently sitting on ice.

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  It works better sitting on ice.

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  Who would have guessed?

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  It's definitely ice cold.

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  And we also have coffee filters with us Subway like

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  like your coffee grounds.

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  We don't want coffee grounds in our coffee.

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  We don't want strawberry in our strawberry syrup water or

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  soap mixture.

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  When we extract the DNA, I'll show you a close

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  up of that right now.

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  Faller supplies.

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  So the banana test tubes and the other items the

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  strawberries, soap, coffee filter, rubbing alcohol, salt.

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  Okay, step one, we're gonna parade that fruit, so I'm

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  being trusted with knives.

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  Now the scientist, that's very important to be trusted with

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  the knives.

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  I don't know if you guys at home or trust

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  with names, but if you wanna be a scientist.

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  Get on that.

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  Ask your parents.

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  Hey, can I chop up the fruit or the vegetables

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  for dinner?

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  They might say yes.

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  They might say no.

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  But if you don't ever ask, you know, you'll never

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  know. I'm gonna go chop, because the more surface area

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  to create the quicker and easier it'll be to puree

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  this route.

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  Now, that's sufficiently chopped.

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  We're gonna put it back in this baggie.

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  Uh, they're all mushy.

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  Mushroom, mushroom, mashed bananas.

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  I don't know about you, but I don't like that

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  sensation of mushiness.

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  Now who in here and watching is Team Banana who

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  thinks the banana committed the crime?

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  So we're gonna go much last night and you can

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  also use the deeper the help curate the girl it

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  provides, like just more pressure spread out.

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  And that much is it even quicker?

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  It's like making banana bread, but not as awesome.

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  Then we're going to do the same with our strata,

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  but that we're gonna see if we can just mush

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  it without taking out, because they're usually more watery.

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  So I don't want to take it out and chop

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  it and create a bigger mess for Mrs Henderson.

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  She does not appreciate my muscles in life.

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  Someone won.

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  The students earlier was talking about how this looks exactly

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  like when you make cheesecake like strawberry cheesecake.

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  And I'm like, Okay, that's cool to know.

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  Yeah, again, I'm using that beaker to pull up to

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  apply more pressure.

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  Right? Both are sufficiently mushed.

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  Let's see what our next step in and extracting this

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  d n a.

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  And finding out who are true crime criminal is or

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  true victim is so first, we have to make some

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  salt water.

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  Mrs. Henderson was gracious enough earlier to heat up some

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  water going to measure out about half a cup.

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  Okay, this is Oh, that's the perfect time.

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  Make sure when you're at home always if you're handling

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  hot stuff to be careful and know the temperature because

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  you don't want to burn yourself, that is not the

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  intended thing you want to do as a scientist, especially

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  to your hand.

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  Use your hand for everything.

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  We're gonna add a teaspoon of salt, and then we're

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  going to start it.

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  Now the salt helps clump with the fruit to like

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  get that DNA to clump.

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  It's pretty cool.

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  It's science.

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  We go Store store stir.

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  Awesome. We're gonna add the water.

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  Mhm. And then I'm gonna repeat that process or my

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  strawberries. So I'm just going to pause the video.

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  Okay, so we are back.

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  I got the saltwater made and I have added to

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  both our bananas and our shared right.

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  So we're gonna go and step three so combined through

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  instead, embrace that I got the saltwater added, No, I

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  just have to combine and mix.

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  Make sure you know the temperature of the water when

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  you're doing this, especially if you're doing in a plastic

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  bag and not a bowl, because that can be of

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  the tool.

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  That's just how painful through a plastic value if it's

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  too hot.

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  Yeah, not fun times.

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  Now I like.

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  Okay, So, like actual crime scene investigators and I'm like,

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  this is like, a true thing.

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  I learned they don't actually like on television.

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  Like with CSS.

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  They get to know the crime that occurred, but in

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  real life, a lot of times they don't actually tell

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  the crime scene investigators what crime is committed.

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  Just hey, extract this D n a or Hey, come

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  take the fingerprints up.

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  Well, in fact, So let's imagine what crime Leave bananas

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  and sure brace we're part of I'm going with bank

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  robbery. Someone seems pretty pumped in and say Seems like

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  something banana and strawberry would go.

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  Or maybe something at the grocery store, because, you know,

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  they're all right at the grocery store.

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  You can definitely comment and tell me what crime you

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  think the first commanded or were victims of.

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  Because what was the best time?

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  One? Was it, like was the criminal?

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  If we don't know?

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  Okay, now this is going to get where it's really

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  fun. We're gonna do our test tube.

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  We're going to get our coffee filters one second while

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  I go grab another coffee.

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  Okay. Handy dandy coffee filter.

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  Put it in first.

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  We're gonna pour.

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  Are sure bring mixture.

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  So I'm being careful and going slow, making sure to

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  not overfill that filter most of its share price.

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  And now it's extracting.

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  Now I'm going to do the same with my banana

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  mush. Oh, sure.

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  Mhm. Oh, no.

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  So I was having so much fun with this.

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  I totally forgot a step, you know, fantasizing about the

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  crimes. He's bananas and servers were criminals and victims in.

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  I forgot to add dish up.

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  So I added my strawberries back to the bag.

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  I'm gonna add my soap.

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  That fresh islands up that was talking about earlier in

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  great detail.

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  It smells very flowery.

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  So adding a good squirt, definitely more than what I

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  would need to wash the dishes.

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  Then I'm gonna gently agitated.

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  I don't want bubbles, but I want to definitely mixed

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  up. Well, that's what helps break down that cell to

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  extract that D N A.

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  That DNA is inside the nucleus, and that needs to

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  be broken down.

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  Yeah, Luckily for us, it's so valuable.

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  So I'm gonna do the same with my bananas.

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  Good squirt.

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  Yeah, agitate it sometimes from okay makes it nice and

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  broken down.

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  Now I can add my we added to that coffee

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  filter and start extracting.

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  So it's important to make sure that you're not spending

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  so much time on, like join the fun things that

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  you don't forget to read those directions and make sure

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  you're following on step by step.

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  And that's why it's important to have it out in

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  front of you now.

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  Do the same as the bananas.

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  Definitely. There's more banana because, well, there was more banana

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  to begin with.

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  Like both filter out.

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  Yeah, definitely.

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  Add to take both a little bit.

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  Yeah. Yeah.

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  Get some that liquid through.

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  I don't need too much to extract the DNA, but

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  I definitely need some.

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  Okay, Okay.

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  We'll give it another 10 seconds, because I have enough,

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  and I'll show you what it looks like.

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  Yeah, you can definitely see the strawberry more than the

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  banana just because of the colors.

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  Yeah. Yeah.

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  Okay. And now I'm just going to set aside some

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  things aside in a cup so they can drain the

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  rest away.

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  And our next step.

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  Let's look at it and making sure we're following those

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  directions. Throw away the coffee filter.

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  We separate the coffee filter.

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  We have banana, we have strawberry.

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  And then our next step, This is where we get

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  to add the rubbing alcohol and the real science begins,

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  So this I'm going to read it.

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  Okay. We're going to take some rubbing alcohol.

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  I got four in, like speaker.

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  Mhm. Yeah.

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  Now, this is where you have to pour very carefully

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  because you want to layer like we did at the

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  beginning of the science beginning of the year when we

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  were doing some of the labs where we had to

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  layer chemicals together and find the different colors.

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  So we're gonna go slowly.

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  Yeah, we got tilted as much as we can.

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  So we create, like a layer.

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  Yeah. Okay.

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  And then I'm gonna do the same thing over here.

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  Notice? I have an angle, and I'm going slowly.

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  So this one, I definitely have, Like, you can tell,

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  the layers is pretty cool.

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  This one is the bananas.

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  You can see a definite layer.

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  Also, you can also see the DNA just instantly coming

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  alive. Well, being extract.

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  Okay, So now, because I'm running slow one time, we're

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  going to go to the next step and we're gonna

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  wait for eight minutes and we are over here, still

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  waiting, but